DECOMPOSITION OF PERFLUOROCARBONIC ACIDS BY PSEUDOMONAS BACTERIA IS CONFIRMED BY DATA ON THEIR GENOMES AND METABOLITES

С.П. Четвериков, Г.Г. Худайгулов, Д.А. Шарипов, С.Н. Стариков

Abstract


Pseudomonas strains 2,4-D and P. mosseli 5(3) designated as UIB-53 and UIB-251 in the Ufa Institute of Biology (UIB) Collection of Microorganisms were isolated from soils contaminated with pesticides and halogen-containing organic compounds and were evaluated for their potential ability to degrade perfluoroorganic acids (PFOA). Whole genome sequencing of their DNA (5.68 and 5.86 bp for 5(3) and 2,4-D strains respectively) showed the presence of genes whose products may be implicated in degradation (dehalogenation) of fluoroorganic compounds: haloalkane dehalogenase (dhaA), H-1 haloacetate dehalogenase (dehH1), fluorine ion transporter (CrcB), and alkanesulfonate monooxyganase (SsuE). After 7 days of cultivation in a liquid medium containing different C7-C10 PFOA species as the sole carbon and energy sources, the strains utilized them completely. LC-MS results suggest that PFOA decomposition leads to perfluorohexanoic acid formation associated with the generation, at different stoichiometric ratios depending on PFOA species, of indicators of fluorine ion mineralization, which were detected using chromatography with conductometric detection. The results suggest that the Pseudomonas strains studied possess potentially high potential for PFOA decomposition, which is confirmed by genomics data.

Keywords


perfluorocarbonic acids, decomposition, Pseudomonas, genome


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References


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DOI: http://dx.doi.org/10.24855/biosfera.v17i1.955

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